The authors have declared that no competing interests exist.
Ascorbic acid is a water-soluble vitamin (Vitamin C) essential for both the plants and animals for the metabolic process. In this study, the liquid chromatography-mass spectrometry (LC-MS) analytical technique was used to characterize the structural properties and isotopic abundance ratio to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on L-ascorbic acid compared to the control sample. The ascorbic acid sample was divided into control and treated parts. Only the treated part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mahendra Kumar Trivedi. The control and treated samples showed a chromatographic peak at retention time (Rt) 1.8 minutes exhibited the deprotonated molecular ion peak at
Ascorbic acid (Vitamin C) is required by both plants and animals for the essential metabolic process. Vitamin C is plenty available in citrus fruits and vegetables, red peppers, potatoes, milk, oysters, animal liver, etc.
The limitation associated with ascorbic acid is that it degrades while cooking, processing, packaging, storage, and cooking of blended foods
The Trivedi Effect®
The L-ascorbic acid (vitamin C) powder sample was purchased from Alfa Aesar, India. Similarly, other chemicals like acetonitrile and ammonium acetate were purchased from Merck India.
The vitamin C test sample powder was divided into control, and Biofield Energy Treated parts. The control sample did not receive the Biofield Energy Treatment but was treated with a “sham” healer who did not have any knowledge about the Biofield Energy Treatment. However, the treated ascorbic acid was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely under standard laboratory conditions for 3 minutes by the renowned Biofield Energy Healer, Mahendra Kumar Trivedi, USA. After that, both the ascorbic acid samples were kept in sealed conditions and characterized using LC-MS analytical technique.
The LC-MS analysis of ascorbic acid was carried out with the help of LC-MS ThermoFisher Scientific, the USA equipped with an ion trap detector connected with a triple-stage quadrupole mass spectrometer. A reversed-phase Thermo Scientific Synchronis C18 (Length-250 mm X ID 4.6 mm X 5 micron), maintained at 25˚C was used. The diluent used for the sample preparation was methanol and water. 10 µL of the ascorbic acid solution was injected, and the analyte was eluted using 80% acetonitrile + 20% 5 mM ammonium acetate pumped at a constant flow rate of 1 mL/min (total run time 10 min). Peaks were monitored at 220 nm using the PDA detector. The mass spectrometric analysis was performed under -ve ESI mode.
The values of the natural isotopic abundance of the common elements are obtained from the literature
% Change in isotopic abundance ratio = [(IARTreated – IARControl)/ IARControl)] x 100 (1)
Where IARTreated = isotopic abundance ratio in the treated sample and IARControl = isotopic abundance ratio in the control sample.
The chromatograms and mass spectra of both the control and Biofield Energy Treated samples of ascorbic acid are shown in
The mass spectra of both the samples of ascorbic acid exhibited the mass of the deprotonated molecular ion M- peak at
The LC-MS spectra of both the ascorbic acid showed the mass of the molecular ion peak at
P(
P(
P(
PM+1,
From the above calculation, it has been found that
The LC-MS based isotopic abundance ratio analysis PM (
Parameter | Control sample | Biofield Energy Treated sample |
PM at |
100 | 100 |
PM+1 at |
5.21 | 6.42 |
PM+1/PM | 0.05 | 0.06 |
% Change of isotopic abundance ratio (PM+1/PM) with respect to the control sample | 23.22 |
PM: the relative peak intensity of the parent molecular ion [M+]; PM+1: the relative peak intensity of the isotopic molecular ion [(M+1)+], M: mass of the parent molecule.
The study confirmed the structure of the sample as ascorbic acid. The isotopic abundance ratio of PM+1/PM (
The impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on ascorbic acid was identified as significant in the isotopic abundance ratio and chromatographic peak area. The control and Consciousness Energy Healing Treated samples showed a major chromatographic peak at retention time (Rt) 1.8 minutes exhibited the deprotonated molecular ion peak at
The authors are grateful to Sophisticated Instrumentation Centre for Applied Research & Testing (SICART) India, Trivedi Science, Trivedi Global, Inc., and Trivedi Master Wellness for their assistance and support during this work.