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Jun 2025 DOI 10.14302/issn.2766-8681.jcsr-25-5555
Natasha Dharmakusumah TaniaCorresponding author
The roof of an HCl fiber-reinforced plastic (FRP) chemical tank failed by accident after it had been operated for less than its expected lifetime. A possible cause is the change in day and night temperature, which lead to the cyclic change in the concentration of HCl vapor in contact with roof part. To investigate this possibility, a detailed analysis on the durability of the material exposed to cyclic solution concentration is required. In this research, vinyl ester-glass fiber composite specimens were exposed to 35 mass % and 0 mass% of HCl alternately at 40°C to replicate cyclic concentration exposure in both the vapor and liquid phases. The mass uptake and penetration depth of HCl as well as the strength loss of the material were investigated. Mass uptake and thickness change in vapor phase shows two times higher than liquid phase, at 3.5% and 6% respectively. This occurs due to differences in the concentration of solutions that diffuse to the specimen in the vapor phase and liquid phase. This phenomenon arises from variations in the concentration of solutions that diffuse to the specimen in both vapor and liquid phases. The diffusion process is facilitated by osmotic pressure, leading to material swelling and contributing to their degradation. The result of flexural strength measurement of specimens indicated a decreasing trend as exposure time increased. The flexural strength decreases by up to 36% due to plasticization. The Cl and water content profile in specimens that obtained from elemental mapping of cross-sections using Energy-Dispersive X-ray Spectroscopy (EDS) are enhancing the study on how materials degrade, which may be a reason for damage to FRP tank roofs.
May 2020 DOI 10.14302/issn.2641-4538.jphi-20-3329
A.J ThatheyusCorresponding author
PG & Research Department of Zoology, The American College, Madurai, Tamil Nadu, India.
Pesticides are applied in agricultural fields for controlling pest population to achieve crop protection. But they cause damage to nontarget organisms and affect the quality of environment including water, air and soil. The present study has been designed to test the efficiency of Pseudomonas stutzerion the degradation of malathion. The bacterial strain was subjected to 50, 100, 150 and 200 ppm of malathion in minimal broth for 30 hours and changes in orthophosphate levels, pH and turbidity were monitored for every six hours. Efficiency of free and immobilized cells were compared for orthophosphate release. Influence of different sugars on degradation was also compared. Degradation of 150 ppm of malathion was confirmed with UV-Visible spectrophotometric analysis and HPLC analysis. The data were subjected to two way analysis of variance and the results are discussed. (Graph 1) Graph 1. Graphical Abstract
Apr 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3282
A.J ThatheyusCorresponding author
PG & Research Department of Zoology, The American College, Madurai, Tamil Nadu, India.
Pesticides applied in agricultural fields for crop protection result in the contamination of the environment. They also affect the flora and fauna as well as the quality of air, water and soil. Hence their remediation is of concern. Among different strategies available, microbial remediation is cost effective and ecofriendly. The present work is designed to test the efficiency of Pseudomonas stutzeri, a bacterial strain obtained from MTCC, IMTECH, Chandigarh, India. After treating with 50, 100, 150 and 200 ppm of methyl parathion, parameters like, orthophosphate released, pH and turbidity changes were monitored upto thirty hours. Degradation of 200 ppm concentration was confirmed by UV-Visible spectrophotometry and HPLC analysis. This strain effectively degraded methyl parathion and it can be used in the remediation of contaminated agricultural fields.
Oct 2019 DOI 10.14302/issn.2639-3166.jar-19-3015
Gupta RajCorresponding author
Centre for Advancement of Sustainable Agriculture, National Agriculture Science Centre Complex, Todapur Road, New Delhi, 110012, India
Monsoon rains provide relief from the sweltering summer heat conditions, replenish depleted profile moisture to breathe new life in soils. With appropriate management of rain water, Indian summer monsoons boost the level of ‘reservoir of life’. Our inability to manage spatial and temporal rainfall variation features of deficit and excess rainfall episodes and their interactions with soil variability is a major cause of uncertainty in agricultural production. In the past, entire focus of national efforts was on rainwater harvesting, storage and distribution through canal networks and greater reliance on ground water pumping to meet immediate crop water demands. These approaches have resulted in wide spread problems of natural resource fatigue and unsustainable water supplies. This paper analyses the complexities of climate change-land degradation-food security nexus and suggests the need for adopting alternate approaches emphasising on in situ conservation of rain water and its efficient use such as to reverse the processes that contribute to land degradation in specific landscapes.
Dec 2017 DOI 10.14302/issn.2640-6403.jtrr-17-1840
Rogina AnamarijaCorresponding author
Faculty of Chemical Engineering and Technology, University of Zagreb
Up till now, chitosan has confirmed its versatile application in skin, cartilage and bone tissue engineering, as well as in drug delivery applications. This study is focused on enzymatic degradation of porous chitosan structures usually designed for mentioned purposes. In vitro degradation was monitored during four weeks of incubation at physiological temperature and in two different media, phosphate buffer saline solution and water. The scaffolds were characterised before and after enzymatic degradation using scanning electron microscopy and infrared spectroscopy with Fourier transformations (FTIR). According to the gravimetric analysis, higher weight loss of chitosan scaffolds was observed in buffered medium with respect to the water. The results implied that the total weight loss obtained in buffer involves physical dissolution of chitosan and lysozyme cleavage of glycoside bond. Importantly, FTIR identification of chitosan scaffolds after enzymatic degradation indicated the absence of lysozyme activity in water, indicating that weight loss is a result of the chitosan dissolution. This finding greatly impacts design of degradation experiments and characterisation of degradation behaviour of chitosan-based materials utilised as implants or drug delivery systems.
Nov 2025 DOI 10.14302/issn.2326-0793.jpgr-25-5573
E. Imiruaye OghenetegaCorresponding author
Advancements in proteomic and genomic technologies have transformed molecular biology by enabling comprehensive analysis of biological systems at the molecular level. This literature review explores the evolution, methodologies, and practical applications of key proteomic and genomic techniques. In proteomics, tools such as two-dimensional electrophoresis, mass spectrometry, Western blotting, Edman degradation, and functional protein microarrays have facilitated high-throughput protein identification, post-translational modification analysis, and biomarker discovery. Similarly, genomic methodologies like PCR, recombinant DNA technology, gel electrophoresis, and Southern blotting have revolutionized gene detection, manipulation, and expression profiling. The review also highlights the interdisciplinary impact of these technologies across clinical diagnostics, oncology, autoimmune disorders, infectious disease surveillance, cardiovascular research, and personalized nutrition. Integrative approaches combining proteomics and genomics are enabling the discovery of novel therapeutic targets, improving disease classification, and advancing precision medicine. Despite current limitations, such as the absence of amplification techniques for proteins and challenges in data interpretation, ongoing innovations promise to bridge these gaps. This synthesis underscores the pivotal role of molecular techniques in deepening our understanding of human biology and accelerating biomedical advancements for improved healthcare outcomes.
Oct 2025 DOI 10.14302/issn.2474-7785.jarh-25-5784
Marks RayCorresponding author
This mini review examines the potential efficacy of applying electromagnetic field therapy for purposes of reducing osteoarthritis pain and other related disease features such as frailty, muscle sarcopenia, obesity, bone loss and fragility, and cartilage derangements and degradation in the older adult. Based on selected English language literature published largely on PUBMED between January 2000 and October 2025, papers describing the impact and potential synthetic and disease modifying impact of electromagnetic stimuli are explored. These data reveal a high degree of promise in fostering joint tissue reparative efficacy trends post electromagnetic stimulation. Its usage may allay the extent of the disease and its degree of disablement, while facilitating function.
Jul 2025 DOI 10.14302/issn.2766-869X.jfd-25-3841
Jangid RenuCorresponding author
Soil is a natural habitat and ecosystem for microorganisms, including bacteria and fungi. Mostly keratinophilic fungi are found in soil, use keratin as a growth substrate, and are essential to the natural degradation of keratin waste. These fungi include dermatophytes, a potential source of infectious diseases in humans and animals and cause dermatophytosis. One hundred thirty-six soil samples were collected from several sites including animal habitats in Ajmer district, Rajasthan, India. The soil samples were used for the study of keratinophilic fungi related to species richness, abundance, and diversity. The Physio-chemical properties of collected soil samples were analyzed and examined in soil microflora for temperature, pH, and macronutrients including nitrogen, phosphorus, and potassium. Ninety-eight soil samples (72%) showed positive results for the keratinophilic fungal isolates. The isolated fungal species belonging to eight genera and seventeen species included Aspergillus niger, A. flavus, A. fumigatus, Chrysosporium tropicum, Microsporum gypseum, M. canis, Fusarium solani, F. oxysporum, F. verticilloides, Trichophyton mentagrophytes, T. rubrum, T. tonsurans, T. terrestre, T. equinum, Penicillium, Mucor circinelloides, and Blastomyces. The temperature recorded for keratinophilic fungi ranged between 25℃ and 37℃ and pH was found in the range of 7.0 to 8.5. The fungal community was dominated by the order Onygenales followed by Eurotiales and Hypocreales belonging to the phylum Ascomycota.
Feb 2025 DOI 10.14302/issn.2326-0793.jpgr-25-5405
Ho Ming-FenCorresponding author
Alcohol use disorder (AUD) is the most prevalent substance use disorder. Excessive alcohol consumption leads to a range of health issues. We set out to identify inflammatory markers linked to alcohol consumption, which might ultimately offer novel insight into genetic underpinnings and have implications for alcohol-associated disease. Alcohol consumption and blood-based multi-omics data were collected by The Mayo Clinic Center for Individualized Treatment of Alcohol Dependence study. Plasma samples from patients with AUD were used for proteomics analysis using the OLINK “Explore Inflammation” panel (n=410). Liver enzymes were also measured. A genome-wide association study (GWAS) was performed to explore the relationship between genetic variants and plasma TREM2 levels. Our findings show thatplasma triggering receptor expressed on myeloid cells 2 (TREM2), a key gene associated with neurodegenerative disease, was the most significant signal correlated with alcohol consumption, and has also been associated with liver enzyme levels in patients with AUD. We identified the rs7232 single nucleotide polymorphism (SNP) in MS4A6A as a key genetic variant associated with plasma TREM2 levels, with the minor allele (A) linked to higher TREM2 levels and increased alcohol consumption, particularly in men. Furthermore, MA4A6A is an ethanol-responsive gene in a SNP-dependent manner, and the variant genotype of the rs7232 SNP was associated with lower expression for MA4A6A due to proteasome-mediated protein degradation. In summary, this study provides insight into the relationship between plasma TREM2 levels, alcohol consumption, and liver function in AUD patients, shedding light on genetic factors underlying alcohol-related diseases.
Oct 2023 DOI 10.14302/issn.2769-2264.jw-23-4694
Alotaibi MajebCorresponding author
This study aims to determine an empirical relationship between the biological and chemical oxygen demands of industrial effluent running through the membrane bio reactor-based Al-Hasa common industrial wastewater treatment Plant. The relationship between these two parameters was investigated over one month (NOV– 2021 to OCT-2022). The values of R² show that its impossible to predict the result of BOD5 based on COD, however the result of r in AUG month shows a strong relation between them. The average biodegradability index over the one year is less than 0.6, showing that effluent is highly polluted, and biodegradation will not proceed. The plant’s performance in terms of BOD5 and COD removal was very poor over the whole year, however, the months AUG, SEP and OCT months shows a satisfactory performance, this is because a large amount of Industrial wastewater entering the plant contains both organic and inorganic components.
Jun 2023
Rifkatu Kambel DogaraCorresponding author
The increasing demand for environmentally-friendly materials has led to a surge in research on the production of biodegradable polymers. In this study, we investigate the synthesis of a biodegradable polymer by graft copolymerization of gum Arabic (GA) and polyethylene glycol (PEG). GA, a natural polysaccharide and PEG, a synthetic water-soluble polymer, were used as the backbone and graft monomer, respectively. The graft copolymerization was carried out using benzoyl peroxide as an initiator and performed under nitrogen atmosphere. The resulting polymer was characterized by Fourier transform infrared (FTIR) spectroscopy, Xray diffraction (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The FTIR spectra confirmed the formation of the graft copolymer, and TGA analysis showed that the copolymer had higher thermal stability than GA. The DTA thermograms indicated two thermal events. The evaporation of water and organic polyethylene glycol components was measured, and the first mass loss was due to the loss of adsorbed and structural water in the gum Arabic, which occurred between 31.87 and 180°C, while the second, corresponding to the pyrolysis of polyethylene glycol functional groups and polysaccharide decomposition, resulted in a 70% mass loss. SEM morphological analysis of gum Arabic showed aggregates of high irregularity in particle shape. The cracks and holes obtained in the Gum Arabic micrograph disappeared from the new gum Arabic-graft-polyethylene glycol, leaving a smooth surface with scattered particles in the image, which was due to the grafting copolymer. From the XRD patterns, the percentages of the amorphous and crystalline phases were determined. The results show that gum Arabic has a 78% degree of crystallinity, whereas gum Arabic-graft-polyethylene glycol has the lowest value of 51%. Biodegradation activity was observed using the fungus Aspergillus flavus on different days on gum Arabic-g-polyethylene glycol. The results clearly showed inhibition zones with a change in the state of the copolymer from solid to liquid from days 8 to 14. These results indicate that the GA-PEG copolymer has potential as a biodegradable material for use in various applications, such as packaging, agriculture, and medical industries.
Aug 2022 DOI 10.14302/issn.3070-3379.jwc-22-4233
Emmanuel EkpaCorresponding author
Biology Unit, Faculty of Science, Airforce Institute of Technology Kaduna
Man’s activity and/ or inactivity continually impedes the environment in many ways. Sometimes, these impediments could be negative with catastrophic consequences. Emission of greenhouse gases, desert encroachment, deforestation, global warming, and general environmental degradation by different human practices poses a lot of threat to this planet. It has therefore become imperative to explore novel ways or technologies in checkmating hazards emanating from these disasters. Biotechnology which simply refers to the use of living organisms or their parts particularly microorganisms to produce materials for human and animal consumption comes in handy. The aim of this paper is to do a brief review on the merits of applying this technology in mitigating hazards arising from climate change. Focus will be centered on agricultural and industrial biotechnology more as a result of their involvement in land and environmental related matters. Manipulation of crops via genetic engineering for varieties with stress resistance, phytoremediation, tissue culture of orphan crops, and so many other biotechnological practices will be examined so as to highlight their role in stemming disasters caused by climate change. In conclusion, the paper will attempt to explain the reason why developing countries like Nigeria should also join the global race in curtailing this menace via her indigenous biotechnology.
Jun 2022 DOI 10.14302/issn.2474-7785.jarh-22-4229
Marks RayCorresponding author
Department of Health and Behavior Studies, Columbia University, Teachers College New York, USA.
Background Osteoarthritis, a serious joint disease, said to represent a generally declining state of wellbeing and function among many older adults has been shown to be affected to a considerable degree by various negative beliefs and inactions rather than degradation alone. Aim This review examines the case of depression as this pertains to the older adult with osteoarthritis of one or more joints. Specifically, the most up to date information on this topic was sought, as care improvements over the past decade have not shown any impactful population wide results. Method Reviewed were relevant 2021-2022 research and review articles specifically pertaining to what is being observed currently by researchers as far as osteoarthritis-depression linkages goes, as these may reveal opportunities for more profound research, and practice-based endeavors. Results In line with 60 years of prior research, it appears a clinically important role for depression in some osteoarthritis cases cannot be ruled out. It further appears that if detected and addressed early on, many older adults suffering from osteoarthritis may yet be enabled to lead a quality life, rather than a distressing and excessively impaired state of being. Those older osteoarthritis cases requiring surgery who suffer from concomitant depressive symptoms are likely to be disadvantaged in the absence of efforts to treat and identify this psychosocial disease correlate. Conclusion Providers and researchers are encouraged to pursue this line of inquiry and begin to map clinical osteoarthritis measures with those that can track cognitive patterns, musculoskeletal, features and inflammatory reactions along with valid depression indicators among carefully selected osteoarthritis sub groups.
Jun 2022 DOI 10.14302/issn.2470-5020.jnrt-22-4217
O. Henderson JeffreyCorresponding author
Department of Science and Mathematics, Judson University, Elgin, IL 60123, USA
Tay-Sachs disease (TSD) is a rare neurodegenerative disorder caused by mutations in the HEXA gene, which encodes the ɑ subunit of the enzyme β-hexosaminidase A. Lacking this key enzyme in GM2 ganglioside catabolism, individuals who are homozygous for HEXA mutations suffer from abnormal accumulation of GM2 ganglioside in brain and nerve cells, ultimately resulting in the progressive deterioration of the central nervous system. TSD is one of three disorders characterized by β-hexosaminidase deficiency; Sandhoff disease (SD) and the AB variant arise by mutations in the HEXB and GM2A genes respectively, which disrupt other points of GM2 ganglioside degradation. Characterized by developmental delay and stagnation, muscular weakness, coordination deficits, seizures, and eventual hearing and vision loss, these three disorders are clinically indistinguishable and occur in three forms defined by age of onset. While there is a much higher incidence of TSD in the Ashkenazi Jewish population, community carrier screening and counseling initiatives have reduced disease prevalence to about the equivalent of non-Jewish populations; however, such efforts have raised ethical concerns in the Jewish community that are increasingly relevant in light of scientific and medical advancements. Currently, treatments for TSD and its related disorders focus on symptom management, with gene therapies and the application of modified CRISPR-Cas-9 technology being explored.
Dec 2021 DOI 10.14302/issn.2639-3166.jar-21-4018
Gupta RajCorresponding author
Centre for Advancement of Sustainable Agriculture, National Agriculture Science Centre Complex, New Delhi, 110012, India
In India, loss of fertility through soil erosion is primarily a summer monsoons mediated phenomenon. Reversing the land degradation processes contribute to water availability, soil fertility maintenance, adapting to climate change and overall food security. Whereas kharif (monsoon/rainy season crop) foodgrain production largely depends on summer monsoons, the rabi season (post-rainy season/winter crop) rainfall is too little to exert a direct influence. In spite of larger acreage under kharif foodgrain crops, total fertiliser consumption during kharif and rabi seasons is comparable. Negative rainfall anomalies (deficit) adversely affected total fertiliser consumption and their use efficiency. Despite significant differences in fertiliser application rates, the response to applied fertiliser nutrients is almost similar in the two seasons. This implies that nutrient use efficiency (NUE) has a ‘manageable’ and an ‘unmanageable’ component wherein 4R practices are difficult to implement under unfavourable kharif weather conditions. Partial factor productivity of fertilizer nutrients (PFPF) has continuously declined over decades mainly because of depletion of soil organic carbon, imbalanced use of nutrients and inability to maintain soil moisture supplies. These observations plus yield-gap analysis permitted us to conclude that past trends of declining NUE can only be reversed through a shift either in sustainable land management practices or enhancing the genetic yield potential/ biomass of crop cultivars or by combining both and making kharif crop planting independent of monsoons rains through direct dry seeding.
Oct 2021 DOI 10.14302/issn.2641-7669.ject-21-3970
Dias Toledo Arruda-Neto JoaoCorresponding author
Linear Accelerator Laboratory, Physics Institute, University of Sao Paulo, São Paulo-SP, Brazil
Microcystins (MCs) are toxins profusely synthesized by cyanobacteria, causing livestock poisonings and endangering human health. We design and execute an experiment to investigate the attenuation (degradation) of microcystins by exposing them to gamma radiation and electron beams at doses of 0 (control), 3, 5, 10 and 15 kGy. The experimental conditions simulate microcystin contamination of aquatic environments; we thus consider (1) microcystins inside whole cells and extracellular dissolved in water, simulated by non-sonicated and sonicated cells, respectively, and (2) two acute microcystin concentrations within water. Toxicity tests of Microcystis aeruginosa detected immobilization (i.e., paralysis) of Ceriodaphniasilvestrii exposed to aqueous crude extracts of irradiated and non-irradiated M. aeruginosa (NPLJ-4 strain) at concentrations of 45 and 90 mg.L-1 (mg dry weight of freeze-dried material), and the results were analyzed using the Trimmed Spearman-Karber statistical program to obtain 48-h EC50, the average effective concentration causing immobility in 50% of organisms after 48 hours. We conclude that electron beams are effective physical agents for toxin attenuation (degradation) and reach 100% effectiveness at 5 kGy and above; their efficiency is two orders of magnitude greater than that of gamma radiation. This new body of information contributes to (1) remediating environmental water sources; (2) designing water/wastewater treatment facilities; (3) combatting chronic microcystin environmental contamination; and (4) inspiring further studies to promote the use of biomonitors (e.g., Cladocerans) to detect and evaluate microalgae contamination.
Jun 2021 DOI 10.14302/issn.2639-3166.jar-21-3822
Benbi DKCorresponding author
Punjab Agricultural University, Ludhiana 141004, India
In South Asia, land degradation is primarily a monsoon mediated phenomenon restricted to 2-3 rainy months. The overall strategy for land degradation neutrality should (i) favour actions that keep soils covered with residues and (ii) plant kharif (rainy season) crop before the onset of monsoons to provide soil cover. Retention of anchored residues provides surface cover, increases microbial activity, carbon sequestration, and availability of nutrients. Surface retained residues reduce root zone salinization, detoxify phytotoxic monomeric Al in acidic soils and enhance the potential for use of brackish ground water in crop production. Residues covers save irrigation water and overcome the ill effects of poor agronomic and water management practices. Early direct dry seeding in surface retained residues has the potential of making kharif season planting independent of the onset of monsoon rains in South Asia and helps reduce acreages of Kharif and Rabi fallow lands. For improving carbon content in Indian soils, perhaps the most important priority is to devise tillage and crop residue management approaches that promote in situ rain water storage and its use for growing more crops. The paper summarises how crop residues fuel and drive soil functions and related ecosystem services and plant growth.
Feb 2021 DOI 10.14302/issn.2689-2855.jan-21-3745
Jana SnehasisCorresponding author
Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India.
Cholecalciferol (vitamin D3) is used nowadays in nutraceuticals regarding the prevention and treatment of vitamin D deficiency and associated diseases. This study was done to analyse the effect of the Trivedi Effect® - Energy of Consciousness Treatment on the physicochemical, thermal, and spectral properties of cholecalciferol using PSA, PXRD, DSC, TGA/DTG, FT-IR, and UV-Vis analysis. For this study, the cholecalciferol sample was divided into control/ untreated and Biofield Energy Treated vitamin D3. The treated vitamin D3 sample received Biofield Energy Treatment (the Trivedi Effect®) remotely for ~3 minutes by Mr. Mahendra Kumar Trivedi, who was located in the USA, while the test samples were located in the research laboratory in India. The treated sample was designated as the Biofield Energy Treated sample. The PSA analysis showed that the particle size values at d10, d50, d90, and D(4, 3) of the treated sample were significantly decreased by 5.80%, 16.49%, 17.52%, and 16.23%, respectively compared to the control sample. However, the specific surface area of the treated cholecalciferol was significantly increased by 7.26% compared to the control sample. Besides, the PXRD analysis revealed that the relative intensities regarding the characteristic diffraction peaks in the treated sample were significantly altered from -42.56% to 22.42%, along with -41.69% to 72.71% alterations in the crystallite sizes, compared with the control sample. Also, the treated sample showed 2.80% decrease in the average crystallite size. The DSC analysis showed a slight increase (0.24%) in the melting point of the treated sample along with 3.68% increase in the latent heat of fusion (ΔH) compared to the control sample. Also, the decomposition temperature of the treated sample was decreased by 0.29%, whereas the ΔH was increased by 5.79%, compared to the control sample. Moreover, the TGA/DTG analysis revealed the significant decrease in weight loss in the 1st and 3rd step of degradation of the treated sample by 18.58% and 89.81%, respectively, along with 1.83% increase in the maximum thermal degradation temperature compared with the control sample. Overall, the thermal stability of the treated cholecalciferol sample was observed to be increased in comparison to the control sample. Thus, the Trivedi Effect® might be used to produce a different polymorph of cholecalciferol, which possesses the improved qualities in terms of appearance, dissolution, absorption, bioavailability, and thermal stability as compared with the untreated sample. Thus, the Biofield Energy Treated cholecalciferol might be used in designing of better nutraceutical and pharmaceutical formulations possessing improved therapeutic response regarding the treatment of vitamin D deficiency associated diseases.
Feb 2021 DOI 10.14302/issn.2377-2549.jndc-21-3722
Jana SnehasisCorresponding author
Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India.
Iron sulphate is used in the treatment of iron deficiency anaemia and other chronic disorders such as heart and kidney diseases. This study has the objective to analyze the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on the physicochemical, spectroscopic and thermal properties of iron sulphate using various analytical techniques. In this, the test compound, i.e., iron sulphate was divided into two parts; one as control (without Biofield Energy Treatment), and the other as Biofield Energy Treated, which received the Biofield Energy Treatment remotely by the renowned Healer, Mr. Mahendra Kumar Trivedi. The PXRD analysis of the Biofield Energy Treated sample showed significant alterations in the range of -39.49 to 301.40% in the relative intensities, and from -15.40 to 33.36% in the crystallite sizes, compared with the control sample. The average crystallite size of the treated sample was also increased by 4.98% as compared to the control sample. The particle sizes in the treated sample at d10, d50, d90 and D(4,3) values were significantly increased by 67.12%, 47.72%, 33.18% and 42.01%, respectively; whereas, the specific surface area was significantly reduced by 38.39%, compared with the control sample. The TGA thermograms showed three steps of thermal degradation in which, the weight loss of Biofield Energy Treated sample in the first and second step was reduced by 5.82% and 16.09%, respectively, while, it was increased by 6.78% in the third step, compared to the control sample. The total weight loss in the treated sample was also reduced by 2.76%, along with slight alteration in the maximum thermal decomposition temperature, compared with the control sample. The DSC analysis showed the decrease in the melting temperatures of the 1st, 2nd and 4th peaks by 8.24%, 19.29%, and 0.61%, respectively, while 4.57% increase in the 3rd peak of the treated sample, compared with the control sample. The latent heat of fusion (ΔH) corresponding to the 1st, 2nd, 3rd and 4th peaks of the treated sample also showed alterations by -92.29, -86.29, 60.92, and 6.37%, respectively, compared with the control sample. The Trivedi Effect®-Consciousness Energy Healing Treatment might produce a novel polymorphic form of iron sulphate having increased crystallite and particle size along with enhanced thermal stability. It may help in improving the quality, safety and stability during the process of handling, storage, and shipment of the iron sulphate with better therapeutic response against iron deficiency anaemia.
Jan 2021 DOI 10.14302/issn.2470-5020.jnrt-20-3619
Chen KepingCorresponding author
Professor, Jiangsu University, Zhenjiang, China
Circular RNAs (circRNAs) are recently rediscovered eukaryotic molecules that form a covalently closed-loop structure through a special type of alternative splicing known as backsplicing. These closed-loop structures are highly stable and resistant to RNase degradation, and are thereby expressed in a tissue-specific and evolutionarily conserved manner, which regulates the expression of proteins and mRNAs that are involved in the metabolic pathways associated with specific diseases. Recent evidence of the ubiquitous expression of circRNAs in cancer under physiological and pathophysiological conditions indicates that dysregulation of gene and protein expression might promote tumorigenesis and carcinogenesis, and that circRNAs have important clinical significance in the diagnosis, treatment, and prognosis of cancer and other diseases. This review provides a brief introduction to the characteristics, formation, and function of circRNAs. Some of circRNAs act as microRNA (miRNA) sponges to regulate the level of transcriptional splicing and the expression of parental genes through the circRNA-miRNA-mRNA regulation axis. We summarize recent progress in above-mentioned circRNAs associated with Alzheimer's disease (AD).
Jan 2021 DOI 10.14302/issn.2328-0182.japst-20-3664
M. Sebaiy MahmoudCorresponding author
Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.
In this literature review, we will introduce most of up-to-date reported methods that have been developed for determination of lamivudine in its pure form, combined form with other drugs, combined form with degradation products, and in biological samples.
Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Tamilvanan NivedharshiniCorresponding author
India
Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (rice bran) as a substrate. The Agricultural soil sample was collected, after the sample were processed for the preliminary and biochemical tests to identification of Bacillus organism. The Guiacol inducer were used for microbial screening of laccase enzyme production. After that microbial screening, various optimization parameters (pH, Temperature, Inducers, carbon and nitrogen sources) are checked for that production of laccase enzyme in mass level. Based on that optimization the bulk fermentation (large scale) (solid state fermentation) were done as a rice bran substrate. The fermentation product was subjected to analyzed the physiochemical properties and purification based on that techniques of Gel filtration chromatography, Dialysis, Ammonium sulfate preciptation. The protein estimation of that product to analysed by lowry’s method.
May 2019 DOI 10.14302/issn.2379-7835.ijn-19-2831
Jana SnehasisCorresponding author
Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), India
Pyridoxine HCl plays an important role in the human body as a coenzyme in the synthesis process of amino acids and neurotransmitters such as serotonin, norepinephrine, aminolevulinic acid, sphingolipids, etc. The objective of this study was to determine the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on the various physicochemical and thermal properties of pyridoxine HCl using various analytical techniques such. The study plan involved dividing the pyridoxine HCl sample into two parts, in which, the first part was not given any treatment (control sample), while the second part was provided the Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Gopal Nayak and named as the Biofield Energy Treated pyridoxine. The particle size values of the treated pyridoxine was altered by -19.51% (d10), -11.92% (d50), 2.46% (d90), and -2.44% {D(4,3)}; whereas, the surface area was significantly increased by 18.92%, compared to the control sample. The powder X-ray diffraction data showed the remarkable increase in the peak intensities and crystallite sizes of the treated pyridoxine in the range from 8.81% to 21.57% and 9.64% to 17.85%, respectively compared to the control sample. Moreover, the treated pyridoxine also showed an increase in the average crystallite size by 13.69%, compared to the control sample. The total weight loss of the treated pyridoxine was significantly reduced by 13.35% during the thermal degradation; however, the residue weight was increased by 29.48% after degradation, in comparison to the control sample. The maximum thermal degradation temperature of the treated pyridoxine corresponding to 1st and 2nd peak was altered by 4.37% and 2.24%, respectively than the control sample. The latent heat of fusion of the treated pyridoxine was significantly increased by 5.89% compared to the control sample. Hence, it was assumed that the Trivedi Effect®-Consciousness Energy Healing Treatment might form a new polymorph of pyridoxine HCl that might be helpful in designing more efficacious pharmaceutical/nutraceutical product due to its better solubility, absorption, bioavailability, and thermal stability than the untreated sample.
Apr 2019 DOI 10.14302/issn.2377-2549.jndc-19-2701
J Mutesasira,Corresponding author
External studies department, Institute of distance Education, Makerere University
Biogas is anaerobic degradation product formed from aqueous slurry of organic waste in a digester. It can be produced from cattle dung,(cd)chicken droppings, decaying leaves, kitchen waste foods(kwf), sewage sludge, slaughter house, goat, pig or sheep manure, Aqueous slurry of 200g/L of mixed or single substrate of cattle dung or/and kitchen waste evolved up to 400mL of biogas at ambient temperatures. The rate of gas evolution reached 5mL/day on the 15th day using 25% cd mixed slurry. The rates of degradation attained in the mixtures were 1.42ml/g for cd; 1.58mL/g for kwf; 1.78mL/g for 75% cd mixed substrate; 1.78mL/g for 50% cd mixed substrate; 1.92mL/g for 25% cd mixed substrate slurries. The comparative rate of biogas formation ranged from1.25 to 1.35 which was in agreement with the range published in literature of 0.8 to 5.5. Biogas can be synthesized efficiently at ambient temperature in Kampala as was done at mesophilic temperatures elsewhere. However, it may be necessary to attempt producing biogas at different pH and temperatures as well as using other substrates and inoculums.
Mar 2019 DOI 10.14302/issn.2643-0282.imsj-19-2672
Fadel Madkour FedekarCorresponding author
Marine Science Department, Faculty of Science, Port Said University, Port Said, Egypt
Recently, annual swarm of invasive Erythrean schyphozoan RhopilemanomadicaGalil, 1990 appeared along Egyptian Mediterranean coasts causing beach closures and fishing problems. The present study conducted survey and field monitoring on R. nomadica during blooming season in the Egyptian Mediterranean coast throughout three consecutive years (2015-2017). Three main features of R. nomadica bloom were addressed; viz starting date, duration and maximum density of aggregation. In 2015, the bloom started on 28 July, and over the following two years the bloom starting date shifted earlier being 19 July in 2016 and 15 June in 2017. The duration of the bloom varied yearly giving the longest duration in 2017 when the bloom continued in high density for a month. The highest density of R. nomadica was about 896 medusae/1000 m3 in 2017. The medusae diameter ranged between 21 to 112 cm. The average bell diameter for each year displayed gradual increasing values over the years. The consistent annual R. nomadica blooming was attributed to the high level of eutrophication and ecosystem degradation occurred along the Mediterranean coast since last decades. The shifting in the annual bloom starting date and duration may reflect the adaptation of R. nomadica to the climate change effect on the Mediterranean Sea temperature.
Feb 2019 DOI 10.14302/issn.2379-7835.ijn-19-2585
Chen Di-YunCorresponding author
Guangdong Provincial Key Laboratory for Radionuclides Pollution Control and Resources, School of Environmental Science and Engineering, Guangzhou University, Guangzhou-510006, China.
With the possibility of the Water-Energy-Food (WEF) Nexus since a long time back, overlooked interlinkages between WEF are getting the chance to be indisputable. Nonetheless, agriculture is responsible for quite a bit of fresh water over-use. Food production further effects the water and energy sectors through degradation of land, changes in overflow, disturbance of groundwater release, water quality, accessibility of water and land for different purposes. The responsibilities of this unparalleled issue include particular parts of the organization around the Nexus. While a couple of papers try to conceptualize the Nexus-Governance, this phenomenal report gives a rich combination of work for further WEF-Nexus ponders and integrative methodologies.
Jan 2019 DOI 10.14302/issn.2639-3166.jar-18-2496
Raj GuptaCorresponding author
Nutrient depletion and imbalanced use of fertiliser nutrients, inappropriate tillage and rain- water management practices often result in land degradation. Declining soil health contributes to climate change through loss in soil productivity, biodiversity, soil carbon, and moisture and ecosystem services. In order to address declining soil health, government of India has launched a soil health card (SHC) scheme aimed at need base use of chemical fertilisers. The paper points out the short-comings in the SHC scheme. Balanced and need base use of chemical fertilizers can be helpful in environmental protection and restoring soil health. The paper identifies potential agronomic practices and production management systems that can reduce our dependence on synthetic nutrients. Integration of soil fertility management domains with computer based QUEFT crop model has the potential of making fertiliser recommendations more domain and crop specific and less cumbersome. For soil health assessment chemical indicators must be integrated with physical and biological properties of the soils which can be predicted through reflectance spectroscopy. For assessing soil health related issues across different agro-ecoregions, there is however an urgent need for building-up more robust soil reflectance libraries.
Oct 2018 DOI 10.14302/issn.2379-7835.ijn-18-2419
Jana SnehasisCorresponding author
Trivedi Science Research Laboratory Pvt. Ltd.,Bhopal, India
Folic acid is essential in the body for the production of DNA and also plays various vital functions within the body. This study on folic acid was done to analyze the results of the Trivedi Effect® on its physicochemical and thermal properties with the help of analytical techniques. The method involves dividing the test sample into two parts. The first part was termed as a control sample, and no treatment was given to it; while the second part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as the treated sample. The study revealed that the particle sizes reduced by 7.01% (d10), 6.53% (d50), 8.37% (d90), and 15.99% {D(4,3)}, thus increased the surface area by 1.72%, in the treated sample compared with the control sample. The PXRD data showed changes in the peak intensities and crystallite sizes ranging from -36.81% to 113.41% and -15.79% to 318%, respectively, thus increased in the average crystallite size by 22.55% of the treated sample compared with the control sample. The TGA analysis of the treated sample revealed a 3.94% increase in total weight loss that resulted in the remarkable reduction in the residual amount by 14.99%, in comparison to the untreated sample. The latent heat of fusion (ΔHfusion) and latent heat of decomposition (ΔHdegradation) were significantly altered by -10.33% and 10.46%, respectively in the treated sample compared to the untreated sample. The study denoted that the Biofield Energy Treatment is a novel approach that could be used to develop some polymorph of folic acid and that might improve its solubility, dissolution, and bioavailability in comparison to the control sample. Hence, the treated folic acid in the nutraceutical/pharmaceutical formulations might be useful concerning better drug performance and efficacy.
Aug 2018 DOI 10.14302/issn.2575-1212.jvhc-18-2167
Buckley PetraCorresponding author
Charles Sturt University, Wagga Wagga, New South Wales 2650, Australia.
A cohort of Pony Club horses was observed for 13 months with data collected from owner-kept diaries and monthly property visits. All horses studied had access to pasture for all or part of the day. Whilst paddock size was seldom limiting, pasture degradation was evident and observed pasture mass and composition varied greatly. Horses’ body weight indices and body condition scores also varied greatly, closely matching seasonal variations in pasture mass. Increments in bodyweight varied in a non-linear fashion with body condition score. Most horses experienced additional year-round hand-fed supplementation and half of the study horses recorded body condition scores greater than 3.5 out of 5. This suggests that the owners of Pony Club horses did not rely on pasture as the sole source of nutrition, even though, based on estimated energy demands, well-managed pasture can meet the energy requirements of Pony Club horses. Furthermore, the fact that owners appeared not to make horse feeding decisions based on body condition score resulting in obesity, raises health and welfare concerns in this cohort of horses. The inappropriate feeding of supplements observed during times of high pasture growth was a concern. Whilst the ideal pasture for Pony Club horses has not been described, it is likely to differ to those considered ideal for broodmares and other livestock because of these unique health risks associated with high levels of non-structural carbohydrate content in improved pasture species. This study highlights the need to educate owners about recognising unhealthy body conditions of horses and better managing pastures and pasture access to reduce the risk of obesity related disorders, whilst at the same time reducing pasture degradation and supplemental feed costs
May 2018 DOI 10.14302/issn.2639-3166.jar-18-2084
Masoero GiorgioCorresponding author
Accademia di Agricoltura di Torino, Torino, Italy
The biofertilization of cropsusing microbial biota in the soil (MBS) is a modern practice that is used to sustain fertility. MBS agents can promote the yield and health of crops, by luxuriating in the shoot as well as in the root systems. Farmers devoted to systematic MBS fertilization are creating a “Symbiotic” (S) form of agriculture, which offers a greater advantage of resilience than Conventional (C) or organic farming. Since MBS is involved in organic matter degradation, hay-litter-bag probes can be used to reflect a global functionality of the active soil, in the short-medium term. It is here shown that the NIRS hay-litter-bag technique, intended not as mass decay but as a quality evolution of the hay probes, can be modelled as a valid footprint of S vs. C soils. A patented MBS was used in eight experiments in which litter-bags from an S treated thesis were compared with equivalent litter-bags from a non-inoculated C thesis. The chemical signature of the S vs. C in the litter-bag composition was a percentage decrease of sugars and fibres. A smart NIRS device was used to discriminate the origin of the S vs. C litter-bags and a sensitivity of 71% (P<0.0001) was obtained. External validations on 37 S farms showed that three NIRS models discriminated the true positive S spectra, with a sensitivity of 90% as single and 98% as compound probabilities The NIRS radiation of the hay-litter-bags confirmed the results of the S vs. C agriculture soil footprint. Moreover, the SCIO-NIR devices also made it possible to connect the S farms in a smart network.
Apr 2018 DOI 10.14302/issn.2690-4829.jen-18-2025
Brumm PhillipCorresponding author
C5-6 Technologies LLC, 5627 Old Oak Drive, Fitchburg, WI 53711, USA
The goal of this work was to clone, express, characterize and assemble a set of soluble thermostablecellulases capable of significantly degrading cellulose. We successfully cloned, expressed, and purified eleven Clostridium thermocellum (Cthe) cellulases and eight Acidothermuscellulolyticus(Acel) cellulases. The performance of the nineteen enzymes was evaluated on crystalline (filter paper) and amorphous (PASC) cellulose. Hydrolysis products generated from these two substrates were converted to glucose using beta-glucosidase and the glucose formed was determined enzymatically. Ten of the eleven Cthe enzymes were highly active on amorphous cellulose. The individual enzymes all produced <10% reducing sugar equivalents from filter paper. Combinations of Cthe cellulases gave higher conversions, with the combination of CelE, CelI, CelG, and CelK converting 34% of the crystalline cellulose. All eight Acel cellulases showed endo-cellulase activity and were highly active on PASC. Only Acel_0615 produced more than 10% reducing sugar equivalents from filter paper, and a combination of six Acel cellulases produced 32% conversion. Acel_0617, a GH48 exo-cellulase, and Acel_0619, a GH12 endo-cellulase, synergistically stimulated cellulose degradation by the combination of Cthe cellulases to almost 80%. Addition of both Acel enzymes to the Cthe enzyme mix did not further stimulate hydrolysis. Cthe CelG and CelI stimulated cellulose degradation by the combination of Acel cellulases to 66%.
Dec 2017 DOI 10.14302/issn.2640-6403.jtrr-17-1818
Manuela BesserCorresponding author
University Witten/Herdecke, Centre for Biomedical Education and Research (ZBAF), Dpt. Of Translational Wound Research
If a wound progressively heals or the healing process is impaired is basically influenced by the surrounding milieu. This is reflected by the wound fluid. Its specific composition triggers the migration, proliferation and differentiation of dermal and epidermal cells which so far was not sufficiently examined in 2D cell culture models. The influence of the different wound entities was analyzed on a newly implemented three dimensional in-vitro model, which improved the transferability to the in-vivo situation. The influence of pooled wound fluids from patients suffering from acute or chronic wounds were investigated within a time period of 10 days after wound application. Histological and immunohistochemical analyses were performed addressing the impact of AWF and CWF on regeneration, such as cell proliferation, fibroblast activity and cell migration. AWF slightly stimulated fibroblast migration while CWF inhibited their activation and migration. The CXCR4- immunopositive population was continuously decreased compared to the control and AWF treatment. The expression of FAP was enhanced under AWF and medium. In keratinocytes CWF massively stimulated cell proliferation initiating on day six after injury. The presence of 10% CWF inhibited fibroblast activation and migration and induced the degradation of the collagen matrix. Keratinocytes were stimulated to proliferate, resulting in healing inhibiting hyperplasia. Transferred to human wounds, no effective wound closure would be achieved because of the de-regulation of pro-proliferative and migration-stimulating factors and a degraded extracellular matrix. This newly implemented 3D study model represents a novel appropriate in-vitro system for studying healing mechanisms and potential therapeutic applications.
Nov 2014 DOI 10.14302/issn.2377-2549.jndc-14-391
Adil Hashmi AtharCorresponding author
Department of Chemistry. Jamia Millia Islamia (Central University), New Delhi-110025, INDIA
Oxidation of 1-Proponol, 1-butanol 1-Hexanol and benzyl alcohol using chloromethylated polystyrene (6% Cross link) DMG (Dimethylglyoxime) copper complex or Poly(S-DVB)-DMG-M Where M= Cu) as catalyst and tert.butylhydroperoxide(TBHP) as oxidant, was studied. The polymer supported Cu Complex was characterized by some physicochemical and spectroscopic methods like elemental analysis IR Scanning electron micrographs (SEM). In the oxidation of 1-propanol, 1-butanol, 1-hexanol and benzyl alcohol. It was found that Product yield of these alcohols corresponding the order 1-Butanal >1-hexanone >Benzyldehyde > 1-Propanone. Kinetic data indicates that catalyst could be recycled without significant degradation of polymer
Jun 2014 DOI 10.14302/issn.2328-0182.japst-13-288
Pradhan N.Corresponding author
Sr. Professor and Head, Department of Psychopharmacology, NIMHANS, Bangalore, INDIA, 560001
ALS is the neurodegenerative disease which is caused due to breakdown in interaction between UBL and rpn1. In this study, we explore the interaction of UBL and rpn1 which is involved in protein degradation. Protein recycling system plays a crucial role in degradation of deformed or damaged proteins. Task of degradation of damaged ubiquitinated proteins is completed by proteasome with the help of ubiquilin2 protein which links 19s proteasome and poly-Ub chain attached to damaged protein. More specifically, N-terminal UBL domain interacts with rpn1 subunit of base complex of 19s proteasome and C-terminal UBA domain interacts with tetra poly-Ub chain attached to damaged protein. In present study, UBL domains are docked against homology modeled rpn1 with the help of Patch dock server. Further the docked structures are refined using fire dock server and best docked structure is chosen having global energy -16.71. Best docked structures are analyzed using swiss-pdb viewer software to show hydrogen bonds between interacting proteins. Here we explore a mutation E6A and P11A in UBL structure with the help of YASARA which is significantly increasing the interaction between interacting proteins in terms of hydrogen bonds.
Nov 2013 DOI 10.14302/issn.2328-0182.japst-12-119
Nath BipulCorresponding author
Department of Pharmaceutical Sciences, Dibrugarh University, 786004, Assam, India
The purpose of the research is to evaluate Sterculiaurens gum as a carrier for oral colon targeted drug delivery system. Sterculia gum has been reported to have wide pharmaceutical applications such as tablet binder, disintegrant, gelling agent and as a controlled release polymer, but it has not been exploited as colon targeting carrier. For evaluation as a carrier for colonic delivery of drugs characterization of gum was done. Microflora degradation studies of gum were conducted in phosphate buffer solution (PBS) pH 7.4 containing rat caecal content under anaerobic environment. Solubility, swelling index, viscosity and pH of the polymer solution were determined. Different formulation aspects considered were: gum concentration (10–40%), concentration of citric acid (10–30 %) on swelling index and in-vitro drug release. The results of the isothermal stress testing (IST) shows no degradation of samples of model drug, azathioprine, in the drug polymer mixture and the core tablet excipients. DSC and FT-IR study has proved the compatibility of the drug with Sterculia gum and other tablet excipients. Microflora degradation study revealed that Sterculia gum can be used as tablet excipient for drug release in the colonic region by utilizing the action of enterobacteria. Sterculia gum exhibits premature drug release in the upper GIT without enteric coating and may not reach to the colonic region. From the study, Sterculia gum as colon targeting carrier is possible via coating with chitosan/Eudragit mixed blend polymers which would provide acid as well as intestinal resistance; but undergo enzymatic degradation once it reaches the colon.
Jan 2013 DOI 10.14302/issn.2329-9487.jhc-12-102
M. R. Lacerdaa CarlaCorresponding author
Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523.
Background: Heart valves share developmental signaling pathways with cartilage and bone. While calcific aortic valve disease (CAVD) has been associated with valve calcification and stenosis, suggestive of osteogenesis, myxomatous mitral valve disease (MMVD) is characterized by net matrix degradation, exuberant deposition of proteoglycan, and valve regurgitation. Methods: We determined the presence of cartilage-abundant proteoglycan, aggrecan; cartilage-specific type II collagen; chondrogenic transcription factor, Sox9; and osteogenic transcription factor, Runx2 in human normal and myxomatous mitral valve leaflets by immunohistochemistry. Results and Conclusions: Myxomatous, but not normal, mitral valves demonstrated sharp focal areas that were abundant in aggrecan, type II collagen, and Sox9. These focal areas co-localized with areas of myxomatous pathologic change on Movat staining. Some cells in these areas had a round and hypertrophic morphology reminiscent of chondrocytes. Runx2 was only weakly present in normal and myxomatous mitral valves. These findings suggest a focal pathologic process in MMVD that mimics chondrogenesis.