Instructions for Authors
Complete guidelines for preparing and submitting your disease research manuscript.
Manuscript Preparation Guidelines
Diseases publishes original research, reviews, case reports, and brief communications advancing disease science. These comprehensive instructions ensure your manuscript meets our publication standards and expedites the review process. Following these guidelines carefully will help your research reach clinicians, researchers, and public health professionals worldwide.
Manuscript Types
Original Research
- Maximum 5,000 words excluding references
- Abstract: 300 words structured format
- Up to 8 figures and tables combined
- Minimum 30 references
Review Articles
- Maximum 7,000 words excluding references
- Abstract: 350 words narrative format
- Up to 10 figures and tables combined
- Comprehensive reference list expected
Case Reports
- Maximum 2,500 words excluding references
- Abstract: 200 words unstructured format
- Up to 5 figures and tables combined
- Minimum 15 references
Brief Communications
- Maximum 2,000 words excluding references
- Abstract: 150 words unstructured format
- Up to 4 figures and tables combined
- Focus on novel findings and methods
Manuscript Structure
Title Page
Include concise title under 20 words, full author names with degrees, institutional affiliations, corresponding author contact details with ORCID identifier, running head under 50 characters, and word count for main text.
Abstract
For original research use structured format with Background, Methods, Results, and Conclusions sections. Include three to five keywords from MeSH terminology relevant to disease research.
Introduction
Provide context for the research question, summarize relevant literature on the disease or condition, identify knowledge gaps, and clearly state study objectives or hypotheses.
Methods
Describe study design, patient selection criteria, diagnostic methods, treatment protocols, outcome measures, and statistical analysis methods with software specifications.
Results
Present findings objectively with appropriate statistical analysis. Use tables and figures to display data clearly. Report effect sizes and confidence intervals alongside p-values for clinical relevance.
Discussion
Interpret results in context of existing literature, acknowledge limitations, discuss clinical implications for disease management, and suggest directions for future research.
References
Use Vancouver citation style with numbered references in order of appearance. Include DOI for all available references. Ensure citations are recent and directly relevant to the manuscript.
Figures and Tables
Submit high-resolution images minimum 300 DPI in TIFF or JPEG format. Include descriptive captions. Tables should be editable format with clear headers and statistical notations.
Ethical Requirements
Human Subjects Research
All research involving human subjects must have institutional review board or ethics committee approval. Include approval reference number in Methods. Describe informed consent procedures and ensure patient privacy protection.
Animal Studies
Research using animal models must comply with institutional animal care guidelines and national regulations. Describe anesthesia, procedures, and humane endpoints. Follow ARRIVE guidelines for reporting.
Conflict of Interest
All authors must disclose relevant financial relationships, consulting arrangements, and potential conflicts that could influence research or interpretation. Declare funding sources with grant numbers.
Authorship Criteria
All listed authors must meet ICMJE authorship criteria demonstrating substantial contributions to conception, design, data acquisition, analysis, drafting, and final approval.
Patient Privacy: Remove all identifying information from images including facial features, unique markings, and hospital identifiers. Obtain written consent for publication of recognizable images. Ensure patient confidentiality throughout the manuscript.
Formatting Guidelines
Prepare manuscripts in Microsoft Word or compatible format with standard margins of 2.5 centimeters on all sides. Use 12-point Times New Roman or Arial font with double-spacing throughout including references and figure legends. Number all pages consecutively and include line numbers for reviewer reference. Use standard abbreviations or define non-standard abbreviations at first use.
Statistical Guidelines
Report statistical methods with sufficient detail to enable verification by readers with access to original data. Specify statistical software with version numbers. For comparative studies, describe sample size calculations and power analysis. Report both p-values and effect sizes with confidence intervals to convey clinical significance alongside statistical significance.
Data Availability
Authors are encouraged to share research data supporting their findings. Include a data availability statement describing how readers can access underlying datasets. Options include public repository deposit with DOI citation, supplementary data files, or controlled access through institutional agreements.
Supplementary Materials
Authors may submit supplementary files including extended datasets, detailed protocols, additional tables, and multimedia content. Files should be under 100 megabytes. Supplementary materials undergo peer review but are published online only.
Revision Submissions
Revised manuscripts should include a detailed point-by-point response letter addressing each reviewer comment. Highlight changes using track changes or colored text. Submit within sixty days of decision notification. Extensions may be requested through editorial office communication.
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